ln the present study 740 specimens collected from susp ected patients in Baghdad including 300 males and 440 females at different age groups. The Results demonstrated that 16% of males and 0.909% females were infected, Three media were tested for preservation of ten isolates of N. gonorrhoeae,these media were LSPQ (LSPQ preservation medium), Yamai (Gelatin-based medium) and TSBG (Trypticase soy broth plus 10% alycerol), the preservation was carried out at -20° C. After six months for storage, the percentage of loss in the viability was (3.244-7.546) and (3.253-8.554) in both LSPQ and Yamai respectively, whereas the % of loss in viability was (27.590-63,982) in TSBG. The colonial types (Tl, T2, T3 and T4)were maintained well in three types of media. However, a slight decrease in frequency was observed in T1-T2 ranging from (2-10%) was observed in both LSPQ and Yamai preservation media, whereas high decrease in frequency of T1-T2 ranging from (5-25%) was observed in TSBG. Antimicrobial susceptibility of the .N. gonorrhoeae indicated that the tested 52 isolates were resistance to clindamycine sulphate, cloxaciline sodium, nitrofurantion, lincocin HCI, metronodazole, orbenin, trimethoprim, and sulphameizol. On the other hand all isolates were sensitive to chloramphenicol base. Penicillinase-producingN. gonorrhoeae (PPNG}was found to constitute (76.93%) of the isolates five different methods0 were employed for detection of .penicillinase. The nitrocefin and the API NHKIT methods were proved to be efficient as compared with congo red, disc diffusion and rapid iodometric methods. Detection of plasmids was performed in the seven-penicillinase producer N. gonorrhoeae (PPNG) which were, tetracycline resistant N. gonorrhoeae. (TRNG) and three non-penicillinase producing N. gonorrhoeae (NPPNG), which were tetracycline sensitive N. gonorrhoeae (TSNG). The results indicated that a correlation between resistance to pencilliri and tetracycline and plasmid contents might exist. Detection of plasmids was performed in four phenotypes(Tl,T2, T3, and T4) of two isolates (118 and 152). The results indicated there is no correlation between the N. gonorrhoeae phenotypes and plasmid contents. MIC values of ten selected N. gonorrhoeae isolates for penicillin, tetracycline, nalidixic acid, ciprofloxacin and norfloxacin were: (0.06-> 32) ug/ml, (0.06-8) ug/ml, (0.125-8) ug/ml, (0.001-0.005) ug/ml and (0.06-0.2) ug/ml respectively. The MIC values did not change after six months of preservation of the isolates. The N. gonorrhoeae isolates were IgAl protease producers when using Audry Norma Medium (ANM) broth culture. IgAl protease was purified with a yield of 5.53% by ammonium sulfate precipitation, ion exchange, and gel filtration chromatography. IgAl protease enzyme was inhibited by 10 mM, 15 mM and 50 mM of EDTA, by 4M and 6M of urea and 1% and 20% of SDS. The obtained results showed that there is no correlation between the plasmid contents of bacterial isolates and enzyme production