Studies concerning colorectal cancer Cell cycle proteins showed that tumor suppressor proteins like: p53, p27, pRb and transcriptional proteins like: NF-KB, play a major role in being a control key for initiation, progression and prognosis of colorectal cancers so, our study focused on Estimation of the tumor suppressor proteins (p53, p27 and pRb) in Iraqi cases of colorectal cancer and nuclear factor kappa b expression in colorectal cancers and also the correlation between each others. Accordingly, a total of 50 consecutive patients with colorectal carcinoma were studied (27 male and 23 female) with a mean age 53.7 years (range between 21 to 82).Each patient was interviewed and his/her paraffin embedded blocks were retrieved.Histopathological data including site, histological type, stage ,and grade of the tumor were also obtained. The paraffin- embedded sections were investigated by immunohistochemical technique for the expression of p53, p27and pRb and, by In situ hybridization for the presence of NF-KB gene. The high expression of p53 and pRb (p=0.01) were observed while p27 was showed non-significant decrease in expression and NF-KB was highly shows a high expression (p=0.01) .highly Significant Correlation was noticed between p53 and pRb in a parallel direction while the opposite happened between p53, pRb and p27 while NF-KB was not correlated to any tumor suppressor protein. In conclusion, this study showed that the left colon is the main site for colorectal cancer cases also, Astller-Coller staging (especially B&C) was seen as significant associated with p53, p27 and pRb while no significant association was noticed with NF-KB.