Diabetes has been largely ignored when assessing male fertility, due to a paucity of studies and inconsistencies regarding the condition‟s impact on semen quality. Therefore, this study was planned to investigate the effects of daily injections IV with GSH (11.37 mg/mice) on healthy and diabetic male mice. The results were compared with the control group and the diabetic non treated male mice group. Each group comprised 6 male mice. The treatment periods were divided into two periods: 10 and 20 days. The studies which have been done for these experimental groups take account of studying the blood sugar tests when the groups of mice were fasting, diabetic, the in vitro fertilization outcomes, the histological changes, the immune localization of the glutathione redox system enzymes GR and GPx in testis and epididymus cross sections, total glutathione assay in different body organs and a comparison between the experimental groups was also made. It finally studied the expression level of GR and GPx in the same organs mentioned before by the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Results of this study are summarized as follows: Glutathione daily IV injected in a period of 10 to 20 days for the diabetic induced male mice significantly high sugar level decreased and enhance the sperm parameters which led to improve the in vitro fertilization outcomes. By contrast the light and transmission electron microscope detections for the histological changes have revealed no drastic changes for diabetic and glutathione treated mice. However, the fluorescent immune localization for GR and GPx5 in epithelium cells in epididymis and testis never changed. Moreover, the oxidative stress in diabetic male mice leads to depletion of total glutathione level in body organs (pancreas, spleen, epididymis and testis). Finally, Total RNA isolation and RT-PCR study increase of expressions of antioxidant enzymes GR and GPx in pancreas, epididymis and testis. Those results lead to a conclusions that glutathione neutralize the oxidative stress caused by diabetes and that lead to deplete the high sugar level to a normal level and the IVF outcomes to a normal levels as in the healthy mice and those effects cause no histological changes in testis and epididymis or alterations in GPx or Gr localization in same organs, high oxidative stress also leads to the depletion of total GSH and increase the expression for the genes of GPx and Gr in (pancreas, spleen, testis and epididymis) and the daily injections with GSH neutralizes that to the normal levels of expressions as in healthy mice.