This work involves synthesis and biological activity of some amino and barbituric acid derivatives via shiff bases. This work is divided into six different parts: Part One: This part involved the synthesis of different shiff bases [1] by reaction of the aniline with benzaldehyde and its derivatives. Part Two: This part involved synthesis of phenoxy acetic acid [II] which converted to phenoxy acid chloride [Ø]. Reaction of the latter compound with shiff bases resulted in part one yields acetanilide derivatives [2]. Part Three: This part involved synthesis of amino acid derivatives [3, 4, and 5] by reaction of acetanilide derivatives resulted in part two with different of L-amino acids (Cysteine, Valine, Phenylalanine) as shown in scheme [I]. Part Four: This part involved synthesis of barbituric acid derivatives [7] by reaction of acetanilide derivatives resulted in part two with guanidine carbonate and diethyl malonate (DEM) as shown in scheme [II]. Part Five: This part involved characterization of the products above by their melting points, elemental analysis and FTIR spectroscopy listed in table 4, 9, and 11. Part Six: This part involved the evaluation of antibacterial activity of the products. These activities were determined in vitro using disc diffusion method against two pathogenic strains of bacteria {Pseudomonas aeuroginosa and Staphylococcus aureus}, as shown in table (4-1).