Processing of optical laser microscope images for material and medical Applications

number: 
503
English
Degree: 
Author: 
Hayder Mahdi Daoud Al-Joboury
Supervisor: 
Dr. Ziad T Al-Dahan
Dr. Muthana J. Al-Khishali
year: 
2001
Abstract:

The Optical Laser Microscope (OLM) provides new information concerning a wide range of specimens. It is particularly advantageous for studying tissues, materials, and devices, including semiconductors. In this work, the mechanism and principle of the OLM is described the laser light enters the objective lens of a microscope through a pinhole. An image of the pinhole is focused on the plane of the object. The beam impinging on the object is reflected from it, and an image of the illuminated spot on the object is focused on the pinhole. The light passes through the (back focal plane) BFP of the objective lens to a CCD camera. The outputs from the CCD camera become maximum when the object is located at the focus of the lens, otherwise the light received at the BFP is defocused. The microscope workstation is developed to provide a powerful image processing system to display and process the OLM images. The modular hardware interface is operated so that the workstation can be easily adapted to control and accept data from the OLM that measured spatially resolved data. The workstation accepts data files in a wide variety of formats, from single line scans to large rectangular image. Two imageprocessing algorithms are designed to accept these formats for medical and material researches and treat images as array of data points, not just pictures.