Genetic,biochemical and bacteriological study on conconut water.+CD

As coconut water is of great importance in different biotechnological and
biological applications, this study involved three main axes which can be represented by genetic, biochemical and bacteriological applications to study the biological characteristics of coconut water and its future applications. Genetic study included studying the ability of coconut water as a suitable medium for the merging of foreign DNA molecules through recombination process and expressing their genes in the new host. The plasmid pEGFP was used as a proof for the completion of binding process and screening the hybrid fluorescent proteins which are coded by this plasmid. The plasmid pEGFP was added to fresh coconut water at different concentrations (0.1μg/ml, 0.081μg/ml, 0.06μg/ml,0.04μg/ml and 0.02μg/ml) in order to detect its ability to merge with DNA from coconut water to examine the ability of water to synthesize recombinant proteins. Results showed that 0.1μg/ml gave positive green color after 4hrs and 5hrs. Biochemical application involved studying the effect of coconut water on liver metabolism in vivo white laboratory female mice were used for this experiment, they were injected intrapretonially with different concentrations of coconut water for different periods of time. Results revealed that coconut water had no significant effect on the levels of GPT, GOT and ALP in female mice when compared to control groups treated with normal saline. The study also involved detecting the possibility of using coconut water as alternative culture medium for growing pathogenic and non pathogenic bacteria isolates and comparing it with traditional culture media. Results showed that coconut water at different concentrations between 10-100% was better culture media in comparison to any culture medium (Nutrient Agar, Brain Heart Infusion Agar, MacConkey Agar, Gauza Agar) depending on the viable bacterial count. Also the sensitivity of pathogenic and non pathogenic bacteria against antibiotics (Chloramphenicol, Penicillin, Gentamycin, Erythromycin, Lincomycin, Trimetheprim, Ciproflaxin and Streptomycin) was tested on coconut water agar culture medium at different concentrations between 10-100% and results were compared with Muller-Hinton agar medium. Results showed that the sensitivity of bacteria against the antibiotics was higher on Muller-Hinton agar medium.