In an attempt to enhance crude oil tolerance in tissue cultures and intact plants of Triticum aestivum L., several experiments were carried out. Callus was induced using embryos isolated from the seeds of the two wheat varieties (Abu Ghraib-3 and Dijila) which were used as explants. Callus was induced and maintained on Murashige and Skoog, 1962 medium (MS) supplemented with (0.5) mg/l kinetin and (2) mg/l 2,4-D. Crude oil was added to the culture medium at different concentrations as a contaminating agent. Results showed that callus fresh weight for both varieties decreased with increasing crude oil concentrations to (590.4, 442.8, 485.7, 288.0, 200.2 and 102.8)mg for Abu Ghraib-3 and (614.7, 4590.0, 434.7, 300.6, 230.2 and 110)mg for Dijila at crude oil concentrations (0, 2, 4, 6, 8 or 10)% in the culture medium respectively. Cells that survived on different concentrations of crude oil for the two wheat varieties were subcultured on oil free MS medium for maintenance. Then they were recultured on MS medium containing different concentrations of crude oil. Results showed that callus fresh weight of the two wheat varieties decreased with increasing crude oil concentrations in culture medium reaching (599.4, 468.9, 427.5, 370.6, 85.5 and 60.0)mg for Abu Ghraib-3 and (632.7, 486.9, 455.4, 393.3, 95.9 and 70.4)mg for Dijila at (0, 2, 4, 6, 8 and 10)% respectively. From the results above it was shown that there was an increase tolerance ability to the low concentrations of crude oil in both of the two wheat varieties (Dijila and Abu Ghraib-3). Results showed that Dijila variety gave better tolerance to crude oil than Abu Ghraib-3 variety. In order to asses the effect of crude oil on seed germination and plant height, seeds were sown in soil contaminated with different concentrations of crude oil. Results showed that germination percentage and plant height increased overtime. However a reduction occurred in these parameters with increasing crude oil levels.