Study the effect of epidermin produced by local isolate of S.epidermidis on antibacterial activity of some agents used in wounds and burns treatment. +CD

This study was included many steps, about epidermin of Staphylococcus epidermidis: extraction, purification and synergistic effect of pure epidermin with some drugs and agents that used in treatment of wounds and burns. Seventy skin samples (swabs) were cultured from many Hospitals in Baghdad, during the period from October/2004 to February/2005. Only five isolates were identified as S.epidermidis, the ability of these isolates to produce epidermin was determinated by growth inhibition against (Staphylococcus aureus and Escherichia coli) using agar spot method and epidermin activity was determinated. All isolates of S.epidermidis have the ability to produced epidermin and shown antibacterial activity toward S.aureus, whereas E. coli was not inhibited. S. epidermidis F isolate have the highest activity. Extraction and purification of S.epidermidis F epidermin, after growth in semi solid media by: cooling centrifugation, ammonium sulfate precipitation, dialysis and gel filtration chromatography on sephadex G-75 column. Biological activity and protein concentration were determined during all steps of purification. Purified epidermin was obtained at a concentration 0.2 mg/ml with activity 50 Au/ml. Synergestic effect of pure epidermin with some agents that used in treatment of wounds and burns.