This study aimed to extract the crude from corn silk using (99.9% ethanol .80% ethanol and water) solvents then study the effect of those extract on inhibition urease enzyme which causes infection kidney stones
• This study included measurement of blood serum tests (uric acid ,glucose, Albumin ,total protein , Globulin) and kidney function tests among (100) patients (60 male and 40 female) of kidney stone in
Baghdad city in Ghazi Al-Hariri Hospital for the period from December 2016 to February 2017. Also, (30) age-matched healthy people (15 male and 15 female) were enrolled as control group .The results of statistical analysis was showon that there is significant increase in urea and glucose. And no significant difference in, creatinine, uric acid, albumin ,total protein and globulin in comparison to that control group.
• In this study was diagnosis of struvite kidney stones by study urinalysis's results (pH, RBC. WBC, crystals) , urine culture , and stone analysis.22% patients with renal stones were found to have infection kidney stones .
• Determination urease activity in urine of 22 patient with struvite kidney stone compare with 22 of control .The results of statistical analysis showed that there is highly significant difference (P < 0.0001) of urease activity in urine in comparison to that control group.
• Extraction of corn silk using (99.9% ethanol, 80% ethanol and water)solvents . Rutin, Kaepferol, and Quercetin were detected in those extracts by HPLC. The results were (0.012mg/L) of Quercetin in 99.9% ethanolic extract.(0.1398 mg/L),(0.15 mg/L), and (0.11 mg/L) of Rutin,Kaepferol, and Quercetin respectively in 80% ethanolic extract . (0.071 mg/L) and (0.091 mg/L) of Rutin and Kaepferol respectively in aqueous extract . It is noticed that only 80% ethanol has an ability to extract such three flavonoids.
• Studying the effect of corn silk extracts to inhibit urease enzyme from Klebsiella Species urease and pure urease enzyme. All such extracts revealed an inhibition of urease enzyme in the Klebsiella Species with (IC50 = 235.9 , 305.5 , and 247.3 mg/L) for 99.9% ethanol, 80% ethanol and aqueous extracts respectively, while standard urease inhibitor exhibits (IC50 =138.1mg/L).for thiourea such extracts were utilized in the inhibition of pure enzyme and they exhibited an efficient inhibition of (IC50 = 133.9, 196.5, and 157.7 mg/L) for 99.9% ethanol, 80% ethanol and aqueous extracts respectively as
compared with standard urease inhibitor of which gives(IC50 = 137.2 mg/L) in thiourea .
• Lastly, study kinetic parameter with and without inhibitors (80 %ethanolic extract ) according to Linweaver- Burk plot . The results showed that the inhibition type is competitive inhibitor mode because
the km is changed, while the V max remains unchanged
