Study the Oxidative Stress Statues in Cardiovascular and Hypertension Diseases in Female Patients

Hypertension (HT) and cardiovascular diseases (CVD) share in that one of the main reasons for them is to increase the oxidative stress, which in turn increases the severity of the disease and exacerbation of symptoms. Reactive molecules produced from oxidative stress, in addition to causing tissue damage by oxidation of biomolecules like DNA, lipids, proteins and sugars; it is lead to the formation of mediators with potent inflammatory effect. The objective of this study was to investigate the some markers of oxidative stress in HT and CVD patients in addition to some biochemical parameters related to these diseases.This study involved 84 female subjects aged between (30-65) year equally divided to three groups, first  and second one belong to CVD and HT patients from Ibn Al-Nafese hospital, while the third one for apparently healthy 28 subjects considered as control group. For each subject in the three groups these markers and parameters were evaluated; malondialdehyde (MDA), 8-hydroxydeoxyguanosine
(8-OHdG), albumin, uric acid, urea, creatinine. Also electrophoretic study on the DNA of patients and control group controlled by placental DNA was done to investigate the possible oxidative stress after Fenton reaction in vitro on it. And study the deletion of Glutathion S transferase mu (GSTM1) gene using polymerase chain reaction PCR. The results were compared to control; Lipid profile show higher significant (p<0.01) levels in both CVD and HT compared to control group. There was a significantly higher (p<0.01) in  MDA, and 8-OHdG levels in both CVD and HT patients, while serum albumin shows significantly (p<0.01) lower levels. Uric acid unexpectedly shows higher levels than control group with (p<0.01) although is antioxidant. The electrophoresis study for  GSTM1 gene show a significant difference (p<0.01) in deletion of this gene. Further there is appositive correlation in deletion of this gene and higher MDA and 8 OHdG and negative with albumin while no correlation with urea, creatinine and uric acid.The elevation levels of oxidative stress markers may be due to oxidative damage of tissues that caused by these inflammatory diseases. It concludes there was a positive relation between oxidation results from these diseases and their developments and suggest increase need to intake of antioxidants as precaution in front of these disease.