This research includes determination of drugs SMX, TMP, CAF, HYO and PAR using derivative spectrophotometry (first, second, third and fourth derivative) were developed for binary mixture by applying zero-crossing technique for pure synthetic mixture and their pharmaceutical formulation as follows:
1. SMX with TMP mixture: SMX was determined by applying 1D&4D teach s at 288.0 and 257.8 nm (zero crossing point of TMP) with linear concentration ranges (2-30) and (2-25) mg/L , r = 0.9996 and r = 0.9992 LOD = 0.750 and LOD = 0.360 mg/L and TMP was determined by applying 4D teach at 251.5 nm (zero crossing point of SMX) with concentration range (2-30) mg/L , r = 0.9995 and LOD = 0.382mg/L . The RSD were 0.255, 0.280 and 1.136 for SMX and TMP respectively and applied for (TRIMOL-400SMX, 80TMP mg) and (METHOPRIM-400SMX,80TMP mg).
2. PAR with CAF mixture: PAR was determined by applying 3D teach at 275.8 nm (zero crossing point of CAF).With linear concentration range (2-35) mg/L , r = 0.9987and LOD=0.445mg/L . And CAF was determined by applying 4D teach at 294.7 nm (zero crossing point of PAR). With linear concentration range (2-35) mg/L , r = 0.9995 and LOD = 0.162 mg/L . The RSD was 0.222 for PAR and 0.130 for CAF and applied for (PANADOL EXTRA-500PAR, 65CAF mg).
3. PAR with HYO mixture: PAR was determined by applying 1D&2D teach s at 297.4 and 303.5 nm (zero crossing point of HYO) with linear concentration ranges (2-30) and (2-30) mg/L , r = 0.9998 and r = 0.9987 LOD = 0.081 and LOD = 0.250 mg/L and HYO was determined by applying 1D teach at 215.9 nm (zero crossing point of PAR) with concentration range (2-25) mg/L , r = 0.9997 and LOD = 0.091mg/L . The RSD were 0.107, 0.400 and 0.342 for PAR and HYO respectively and applied for (SPAZMOTEK PLUS-500PAR,10HYO mg).
This thesis has mainly been structured in three different chapters, each one containing the following information:
Chapter one provides a short historical review with the analytical performance characteristics of UV-visible are described. The applications of UV and DS in pharmaceutical and SMX, TMP, CAF, HYO and PAR analyses and their mixture .the general and specific objectives of thesis are reported.
Chapter two corresponds to the experimental part. Reagents, instruments, procedures and detail protocols for the preparation of standard solution and pharmaceutical sample which used in this study are reported.
Chapter three contains the experimental results and discussion that lead to the possibility of successful applications which used DS to determine the concentration of each material in drugs.
