Polyamines, spermidine and spermine constitute a group of cell components that are important in the regulation of cell proliferation and cell differentiation.The enzyme superoxide dismutase catalyzed the breakdown of superoxide anion (O2-) and provides the first line of defense against oxygen toxicity. This work is an attempt to look for possible biochemical marker and causes of breast cancer based on the above polyamines.The levels of spermine and spermidine and superoxide dismutase levels were measured in serum of two women groups. Group one contains (66) breast cancer patients while the second group contains (20) healthy women used as controls.Blood samples from the two groups were prepared for quantitative determination of polyamines using high performance liquid chromatography (HPLC), while SOD was determined using uv-vis spectrophotometer.It has been found that spermidine is significantly increased (P<0.05) in patients with breast cancer (0.184±0.037) compare with normal subjects (0.14±0.01), also spermine level was significantly higher (P<0.05) in breast cancer patients (1.105±0.379) compared with control (0.22±0.08) and with spermidine.SOD level was significantly lower in breast cancer patients (330.141±54.527) when compared to the control group (535.875±39.855) with (P<0.05).From the result of the present study, it can be concluded that detection of polyamines and superoxide dismutase in serum were useful and valuable as diagnostic marker for breast cancer
