Extraction of Medicinal constituents from two Iraqi Herbs Datura stramonium and Atropa belladonna -A chemical study-

number: 
1058
إنجليزية
department: 
Degree: 
Author: 
Bassam Faron Abed Al-Farhany
Supervisor: 
Dr. Shahbaz A. Maki
Dr. Muhannad J. Mahomod
year: 
2005

In this work two Iraqi plants (Datura Stramonium and atropa belladonna) has been studied. Extraction and isolation of atropine from these plants were investigated. The extraction was done using methanol as a solvent using soxlet apparatus. Atropine was identified by melting point which was found to be (113-116 o C) in Atropa belladonna and (114-118oC) in Datura stramonium which was in good agreement to the standard value (114-116C0).  F.T.IR, UV-Vis, HPLC and TLC achieve the presence of atropine. HPLC analysis shows New Flavonoid compounds in the two plants in small concentrations. Rutin and Qurecetin were separated in Datura stramonium while Catechin was found in Atropa belladonna. TLC also confirmed the presence of these compounds by comparison the Rf  values of each extracted compounds with the standard Rf  values. Biological activity was determined using disc diffusion method with two strain of pathogenic microorganisms viz., Staph.aureus and E.coli. These results obtained using different water extract concentrations (5µg/ml, 10µg/ml, 15µg/ml, and 20µg/ml) showed measurable activity against the two types of bacteria.