Low level lasers are used to treat different muscle injuries due to their good response to the photo-bio-stimulation effect. In this thesis, the effect of continuous (CW) and pulsed laser diode (785nm) and Nd:YAG laser (1.064nm) on the healing process of practically managed skeletal muscle trauma has been successfully studied. Standard impact trauma has been introduced by using a specially designed mechanical device. The impacted muscle left for three days for complete development of blunt trauma. After that it was irradiated by five laser sessions for five days. Tissue samples of crushed skeletal muscle have been dissected from the injured irradiated muscle and bio-chemically analyzed for the regeneration of contractile and collagenous proteins using Lowry assay for protein determination and Reddy and Enwemeka assay for hyroxyproline determination. The results showed that both lasers stimulate the regeneration capability of traumatized skeletal muscle. The CW and pulsed diode laser showed better results in accelerating the preservation of the normal tissue content of collagenous and contractile proteins and controlling the regeneration of nonfunctional fibrous tissue. This study proved that the healing achieved with laser treatment is faster than the control group by (15-20) days.