The current study aimed to investigate the molecular defect causing the manifestation of gouty arthritis , the relationship between kidney function and the gout and , study the relationship between white blood cell count and gout in a sample of Iraqi patients .The study included 35 patients suffering from gout during the period from January 2015 to June 2015 at Al- Karamah and Al-Yarmouk teaching hospitals .20 healthy subjects were selected to represent the control group. The mean ages of the patients were 34 -63 years and the mean ages of the control were 25 - 40 years The personal information's for each patient was obtained , including name , age ,gender, weight , length , medications and family history of gout . Uric and creatinine tests were performed to study relationship between the disease and kidney function .White blood cells was counted to investigate the inflammation status .DNA extraction was performed from whole blood samples. After DNA quantification, the DNA samples were used for polymerase chain reaction (PCR) amplification .Agarose gel electrophoresis of PCR product showed the presence of band with size of 300 bp, 400bp, 600bp, 253bp and 296bp. After amplification of SCL22A12 gene that lead to gout.
