number:
1348
إنجليزية
College:
department:
Degree:
Supervisor:
: Dr. Abdul Kareem Hameed Abd
year:
2006
- A total of (150) samples were collected from clinical specimens including (Burns and Wounds) belongs to patients of both sexes and of different ages hospitalized in Al-Kadhumia, Baghdad and AL-Yarmook hospitals in Baghdad during the period from 1-3-2005 to 15-5-2005. Only 50 (33.3%) isolates were identified as Pseudomonas aeruginosa by using Morphological, Physiological and Biochemical tests and hundred (66.7%) isolates indicated the presence of different genera includes P. fluorescence (20%), E. coli (27.7%), Bacillus (11%), Proteus (6%), and Klebsialla (2%).
- Susceptibility of isolates to (20) antibiotics was also tested using disk diffusion assay. Imipenem and Amikacin were to be the most effective antibiotics with no resistance at all while the other antibiotic were less effective.
- Ten isolates were selected according to their pattern of resistance as those showing multi-drug resistance and tested to specify their minimum inhibitory concentration for (Amikacin, Gentamicin, Ceftazidime, Pipracillin and tetracycline). Amikacin was found having the lowest MIC comparing with others.
- This study also include in-vitro effects of various combinations of five types antibiotics (Amikacin, Gentamicin, Ceftazidime Piperacillin and Tetracyclin, ) against the ten Pseudomonas aeruginosa isolates that were found sensitive to each of these antibiotics. Among combinations, the combination of ß-lactam antibiotics with amikacin was found to be the most effective combination that inhibits the growth of Pseudomonas aeruginosa. In which the MIC of Ceftazidime and Pipracillin alone when the isolates no. (1, 2, 20 and 11) were tested was (4, 2, 4 and 23 µg/ml) respectivly while MIC of Amikacin alone in the same isolates was (4, 0.5, 2 and 2 µg/ml), after combination the MIC of Ceftazidime and Pipracillin for these isolates became (0.25, 0.125, 0.25 and 2 µg/ml) respectively while the MIC of Amikacin become (0.25, 0.125, 0.03 and 0.25 µg/ml) respectively.
- The effect of EDTA when it was combined with antibiotics also tested against the ten isolates of P. aeruginosa using disk diffusion assay after soaking the antibiotics disk in EDTA. Results showed that EDTA increases the effect of antibiotic against P. aeruginosa isolates especially when it was combined with aminoglycoside antibiotics.
- Another part of the study includes the using of Lactobacillus acidophilus (LAB) as probiotic to inhibit the growth of Pseudomonas aeruginosa. Minimum inhibitory concentration (MICs) was determined for the three-fold concentrated filtrate of (LAB) against P. aeruginosa. Results showed that (4/6) (filtrate: medium) considered as the MIC of LAB filtrate that inhibit the growth of P. aeruginosa The plasmid profile for the (2) most resistant isolates was studied. The result showed that these isolates contain a small plasmid DNA bands approximately in the same size
