Four samples were taken from the leaves and the flowers of jojoba plant. Extraction solution were prepared from leaves and flowers of the two plant sexes, Total protein was studied using Bradford procedure during three weeks (the last two weeks of July and the first week of August 2004) for the two sexes and conventional differences were recorded between them, also the Isozymes variation was studied between the sexes for the following enzymatic systems:-
1- Glucose 6 Phosphate Dehydrogenase (G6PD).
2-Glutamate Oxaloacetate Transaminase (GOT).
3- Malate Dehydrogenase (MDH).
4- Esterase(EST).
Isozyme variation was detected between the two sexes of the plant in which leaf samples before and during flowering were tested for the first two enzymatic systems (mentioned above), while the third enzymatic system was tested before flowering (leaf samples only) and the fourth enzymatic systems was studied in the leaves and flowers during the flowering time, the test was done using horizontal gel electrophoresis technique to detect the isozyme variation.
The second enzymatic system was purified from the extraction solutions of the two sexes using the ion exchange technique with cellulose methyl cinamate (CMC) and the protein concentration was studied before the purification in the crude samples , after the precipitation by salts (ammonium sulphate) and after the purification by the ion exchange technique.
The enzymatic extraction solution from the two sexes of jojoba was injected in two groups of hosts (14 rabbits each) .After three weeks, 10 healthy rabbits were selected from each group and serum was taken from each host and tested against Ags from both mature and newly formed plant leaves.
Statistical analysis was conducted using T-test to detect statistical differences between the means. The results show no conventional differences found while differences in the specific activity of enzyme and the protein concentration of the crude solution were recorded (between the two sexes).
Several kinds of terpens, tepenoids, eugenols and coumarin substance were studied in both sexes using high performance liquid chromatography in which differences were indicated as similar and intermediate and acute differences
