This study was included many steps, about epidermin of Staphylococcus epidermidis: extraction, purification and synergistic effect of pure epidermin with some drugs and agents that used in treatment of wounds and burns.
- Seventy skin samples (swabs) were cultured from many Hospitals in Baghdad, during the period from October/2004 to February/2005. Only five isolates were identified as S.epidermidis, the ability of these isolates to produce epidermin was determinated by growth inhibition against (Staphylococcus aureus and Escherichia coli) using agar spot method and epidermin activity was determinated. All isolates of S.epidermidis have the ability to produced epidermin and shown antibacterial activity toward S.aureus, whereas E. coli was not inhibited. S. epidermidis F isolate have the highest activity.
- Extraction and purification of S.epidermidis F epidermin, after growth in semi solid media by: cooling centrifugation, ammonium sulfate precipitation, dialysis and gel filtration chromatography on sephadex G-75 column. Biological activity and protein concentration were determined during all steps of purification. Purified epidermin was obtained at a concentration 0.2 mg/ml with activity 50 Au/ml.
- Synergestic effect of pure epidermin with some agents that used in treatment of wounds and burns. Studied for this purpose drugs and agents was used (with active compounds): Samacyclin (Tetracyclin HCL 3%),Nifusin(Nitrofurazon), Silverin (Silver sulphadiazine 1%), Acetic acid 6% and Iodine 10%. The effect of these agents (alone) with concentration 100% and with epidermin 50% (v/v), were determined by using agar diffusion method against S.aureus. Results showed, Samacyclin was the most effective agents, Nifusin was the second, and Silverin, acetic acid 6% and iodine 10% were the least effective agents.
- Plasmid profile was determined for all S.epidermidis isolates. Results showed: The isolates containing a small plasmid bands approximately in the same size compared with each other. Curing results (by using ethidium bromide), showed that epidermin production was carried on plasmid not on chromosome, and the plasmid code for antibiotic resistance gene also.
