Induction of Somatic Embryogenesis in Solanummelongena L

This project was designed to induce somatic embryogenesis on different explants dissected from Solanum melongena L. plant. In this study, calli was induced on stem segments grown on MS (Murashige and Skoog, 1962)medium supplemented with NAA at 8mg/l and Kin at 0.1mg/l, Decreasing the supplemented agar to 5g/l increased calli induction significantly in the rate and time of induction. Calli initiated on stem segments was photographed under light microscope after one week on MS free of PGRs which shown embryos at globular stage. Decreasing the
agar concentration accelerated embryogenesis induction Shoots were developed from embryogenic calli after 30 days on MS free PGRs medium, then transferred to MS medium supplemented with 0.2mg/l NAA and 0.1mg/l BA where roots developed after 30 days. Aِnther culture was maintained on liquid R medium (Dumas de Vaulx et al., 1981) in a cell suspension supplemented with 0.02mg/l 2,4-D and 0.01mg/l Kin at 100rpm in a shaker incubator and photographed under light microscope after two weeks. Embryos at the globular stage were seen and counted in embryogenic calli and the result was 2977000embryo/ml. Embryos cell
suspension count was 3550000embryo/ml.. Viability test was carried out for embryos cell suspension and the viable embryos percentage was 72%.