Isolation and Identification of Klebsiella Accompanying Accident Wounds and Treatment with Probiotics

number: 
2718
إنجليزية
Degree: 
Author: 
SABA RAAD JA'FAR
Supervisor: 
(Professor) Dr. AbdulWahid B. Al-Shaibani
(Assistant Professor) Dr. Sadeq A. Al- Mukhtar
year: 
2011

 A total of (100) samples were collected from patients (referred to three hospitals in Baghdad) suffering of accident wounds included traumatic wounds, foot ulcer and burns. The samples were collected from both sexes during the period 10/12/2009 – 14/3/2010. A total of 22 isolates of Klebsiella were identified depending on cultural, microscopic and biochemical characterizations. Other bacteria obtained were identified as Escherichia coli, Pseudomonas spp., Enterobacter, Proteus and Citrobacter in numbers 25, 23, 6, 5 and 2, respectively.  Biochemical and Api-20E system identification revealed that all Klebsiella isolates were belonged to the species K. pneumoniae.  Antibiotic susceptibility of K. pneumoniae isolates against (15) of all commonly used antibiotic was determined through disc-diffusion method. After that, well-diffusion method was used to investigate the resistance of K. pneumoniae isolates toward six selected antibiotic  A total of (100) samples were collected from patients (referred to three hospitals in Baghdad) suffering of accident wounds included traumatic wounds, foot ulcer and burns. The samples were collected from both sexes during the period 10/12/2009 – 14/3/2010. A total of 22 isolates of Klebsiella were identified depending on cultural, microscopic and biochemical characterizations. Other bacteria obtained were identified as Escherichia coli, Pseudomonas spp., Enterobacter, Proteus and Citrobacter in numbers 25, 23, 6, 5 and 2, respectively.  Biochemical and Api-20E system identification revealed that all Klebsiella isolates were belonged to the species K. pneumoniae.  Antibiotic susceptibility of K. pneumoniae isolates against (15) of all commonly used antibiotic was determined through disc-diffusion method. After that, well-diffusion method was used to investigate the resistance of K. pneumoniae isolates toward six selected antibiotic  A total of (100) samples were collected from patients (referred to three hospitals in Baghdad) suffering of accident wounds included traumatic wounds, foot ulcer and burns. The samples were collected from both sexes during the period 10/12/2009 – 14/3/2010. A total of 22 isolates of Klebsiella were identified depending on cultural, microscopic and biochemical characterizations. Other bacteria obtained were identified as Escherichia coli, Pseudomonas spp., Enterobacter, Proteus and Citrobacter in numbers 25, 23, 6, 5 and 2, respectively.  Biochemical and Api-20E system identification revealed that all Klebsiella isolates were belonged to the species K. pneumoniae.  Antibiotic susceptibility of K. pneumoniae isolates against (15) of all commonly used antibiotic was determined through disc-diffusion method. After that, well-diffusion method was used to investigate the resistance of K. pneumoniae isolates toward six selected antibiotic