Treatment of Salmonella typhimurium with Probiotics to Evaluate it Histopathological and Immunological Effects on Mice

This study was aimed to investigate the protective effect of some probiotic microorganisms in mice infected with Salmonella typhimurium. A total of (119) samples including food, stool, and vaginal swabs were collected from various regions of Baghdad city. Lactobacillus spp. and Saccharomyces spp. were isolated from the samples to be used as probiotics. Twenty isolates of Lactobacillus spp., 17 of Saccharomyces cerevisiae and one isolate of Saccharomyces boulardii were obtained from the samples. Nine isolates of Lactobacillus and eight of Saccharomyces isolates were found to possess inhibitory effect against S. typhimurium )in vitro(.
The most efficient isolate of each of Lactobacillus (Lb. acidophilus Gb9) and Saccharomyces (S. cerevisiae Fs28) were selected to evaluate their effects on the mmunological (total and absolute count of leucocytes, phagocytic index, and total immunoglobulin level) and histopathological (intestine, liver, and spleen) aspects of albino male mice infected with S. typhimurium. For this purpose, 35 mice were divided into 7 groups before subjection to various treatments.