The effects of some fat soluble vitamins and sunflower oil on reproductive performance and the level of serum total cholesterol in female mice.

Antioxidants are chemical compounds that can delay the start or slow the rate of lipid oxidation reaction in food system. Antioxidants considered one of the best protection agents for the body against the side effects of free radicals, enhancing growth factor, improvement of fertility performance and reducing level of total serum cholesterol. Therefore, in this study was planned to investigate effects of orally intake, not exceeded the recommended daily allowance (RDA), of some Antioxidants involving fat soluble vitamins (A and E) and sunflower oil, on the body weight, outcomes of in vivo and in vitro fertilization,, level of total serum cholesterol and histological changes of uterine horns and ovaries of one hundred and eighty healthy female mice were divided into three major groups according to period of antioxidant administration involving 7, 14 and 28 weeks. Each major group was subdivided into five minor groups according to types of antioxidants involving vitamin A, vitamin E, vitamins A+E, positive control (sunflower oil) and negative control. Results of this study are summarized as fallow: Body weight increased for mice treated with vitamin E for 7, 14 and 28 weeks, this group share the same elevation for mice treated with vitamins A+E treated for 14 weeks. Hormonally stimulated-mice have higher absolute weights of reproductive organs than the natural cycle-mice. For the three treatment periods, administrations of different vitamins have better results than the negative control. While results recorded for absolute weights of ovaries and uterine horns for the group treated vitamins A+E for 14 weeks Treated groups with different antioxidants record better results in the pregnant weights and litter size than the negative control group for the three treatments periods. However, the best result was recorded for the outcome of in vivo fertilization for mice treated with vitamins A+E and vitamin E for 14 weeks. Vitamins A+E-treated mice group show the best outcome of VF, especially in the total oocytes collected from this group treated for 14 weeks, but the best percentages of IVM and IVF were recorded for mice group administered vitamins A+E for 7 weeks. While the lowest percentage of abnormal embryonic development was assessed for mice group administered vitamin E for 14 weeks. 5. Histological study appeared that the uterine horns and ovaries of hormonally stimulated groups treated with antioxidants have much better features in both longitudinal sections than the control groups for natural cycle- and/or hormonally stimulated-mice. These uterine features involve thickness of epithelial layer, diameter of uterine horns and uterine glands distributed along the uterine horns. Also, it was reported that hormonally-stimulated mice treated with vitamin E and vitamins A+E for the three treated periods were the best features. Moreover, best results for number and diameter of growing follicles Graafian follicles and corpus luteum were reported for hormonally stimulated-mice treated with vitamin A and vitamins A+E for 7 and 14 weeks, while, administration of vitamin E have best results
for number and diameter of corpus luteum for the three treated periods. 6.Long-term treatment with antioxidants reduces the level of total serum cholesterol than for number and diameter of corpus luteum for the three treated periods. 6.the negative control group and the lowest level of total serum cholesterol was observed for the positive control group after 14weeks.