This project is aimed to study the inhibition effect of wheat germ extract on some microorganisms, cancer cell line, and atopic dermatitis (eczema). The quantitative and qualitative detections of methanolic extract and methanol chloroform method extract by using gas chromatography–mass spectrometry (GC-MS) technique were achieved to detect the active groups. Results showed the presence each of linoleic acid (with an percentage of 40%), oleic acid and octadecanoice acid, hexadecanoic acid which might play very important role in all bioactivity evaluations in this project.The antimicrobial effect of wheat germ extracts (methanol and chloroform methanol) were tested on twelve strains of Gram positive and Gram negative bacteria strains in addition of two fungal strains to find the suitable minimum inhibitory concentration (MIC) the extracts were active and had an antimicrobial activity on almost strains tested MIC ranged between 2.5 to 0.165 mg/ml.The cytotoxicity activity effect of crude extract on cancer human Leukemia cell line (K562), breast cancer cell line (MCF-7) and on normal cell line (Fibroblast).Results was recorded that a significant cytotoxic activity of the extracts in cell lines during the 72 hr. exposure time at concentrations of (900 , 450, 225 and 112.2) μg/ml as compared to normal cell line fibroblast.A formula of ointment from wheat germ extracts were prepared and tested topically as a cure treatment for mice with atopic dermatitis (eczema). Both extracts formula; 20% and extract alone proved curing