Quantitative histochemical study of skeletal muscle fibre types.

number: 
173
إنجليزية
department: 
Degree: 
Imprint: 
Medicine - Anatomy
Author: 
Sahar Hussein Abd Al-Rasool
Supervisor: 
Dr. Anam R. Al-Salihi
year: 
1995
Abstract:

This study is a trial to quantitate histochemical enzymatic reactions in skeletal muscle fibres aiming to reach an objective quantitative way for skeletal muscle fibre typing. A histochemical study was performed on alpha-naphthyl-acetate esterase and succinate dehydrogenase in quadriceps muscle for both human and mouse. The color of the final reaction product was determined visually and fibre typing was performed accordingly then the F.R.P. absorption curves were obtained for the enzymes employed in this study. The wavelength of maximum absorbance for esterase was 425 nm and 550 for succinate dehydrogenase. Measuring the extinction for skeletal muscle fibres and the statistical analysis of these values showed that the optical density of F.R.P. for type I fibres was 1200.91+ 229.57& For type II 957.57+134.52 in human muscles and 1689.26 +123.67, 227.5+ 63.5 respectively in mouse muscle. As for F.R.P. of SDH the optical density for type I fibres was 192.49+48.39 and for type II fibre is 93.4+27.33 for human and 489.39+ 97.14, 84.72+17.85 respectively for mouse muscle fibres. Using the above mentioned histochemical enzymatic parameters demonstrated the presence of intermediate reacting fibres.The frequency distribution curves for the O.D.for different fibre types showed the presence of secondary groups within the visually detected distinctive groups. The SDH reaction gave a more precise differentiation between different fibre types than the esterase reaction which displayed cluster groups( for different types ). This study is an objective cornerstone for application of quantitative histochemistry in skeletal muscle fibre typing and it facilitates the use of this method in research and clinical examination.