Two hundred thirty five total cases were studied and analysed for the presence of rubella specific IgM antibodies by using ELISA technique. They were divided in to :- 1- 110 newborn infants and children with one or more congenital defect(s). 2- 100 women, they were divided in to two groups :- A-50 women with recent abortion or stillbirths they have one or more risk factor(s) which include :-history of rash during first trimester of pregnancy; contact with rash; flu-like illness; this group of women were not vaccinated against rubella virus. B-50 women in the first and second trimester of pregnancy with no history of any risk factors. This group was considered as control group. 3- £5 children suffering from acquired rash and occipital lymphadenopathy..Possible rubella infection. IT WAS FOUND THAT :- 1- No one out of 110 newborn infants and children were positive for rubella specific IgM antibodies by ELISA assay. Their age group ranges from newborn period (one dayjto 15 month • 2- The prevalence of rubella recent infection among studied women was 3 out of 100 women (3%) , the mean age of these women was 25 years. A-The prevalece of rubella specific IgM antibodies among recently aborted women and women with stillbirth was 2 out of 50 (4%) with the mean age of 27 years. B-The prevalence of rubella specific IgM antibodies among first and second trimester pregnant controls was 1 out of 50 (2%) . 3- The two povitive cases in the risk group have two or more risk factors for each. The first one gave a history of flu-rash illness during early pregnancy period; the second women gave a history of flu-like illness. Both of them were nonvaccinated against rubella virus. 4- The prevalence of rubella specific IgM antibodies among the selected group of children with acquired rash and occipital lymphadenopathy was found to be 1 out of 25 (4%), their age groups range from 6 months to 5 years. 5- Two different ELISA tests were used for detecting rubella specific IgM antibody : Clark and Organon ELISA assay. Both systems were sensitive to all positive cases.