The study included identification of sixteen P.aeruginosa isolates, which were isolated from burns. The antibiotic sensitivity pattern was examined for all the isolates. The results showed that from the sixteen isolates, fourteen were resistant to all antibiotics used in the study. Only one strain was sensitive to kanamycin and another strain was sensitive to tobramycin. In addition to P.aeruginosa isolates, four multiresistant bacterial strains (E.coli Z53 & Z149 and K.pneumoniae Z15 & Z29 ) isolated in previous study from patients with urinary tract infection were also used in this study. The antibiotic resistance pattern of these strains was examined. The minimum inhibitory concentrations MICs of three curing agents (Sodium dodecyl sulphate SDS, ethidium bromide and acridine orange) used in this study was determined using P.aeruginosa Nl & N3, E.coli Z53 & Z149 and K.pneumoniae Z35 & Z29 as assay organisms. The results indicated that the subinhibitory concentration for each curing agent varies from strain to another. The curing percentage and efficiency of each curing agent was ; determined. From treatment with SDS no cured cells were obtained from \aeruginosa (Nl & N3), K.pneumoniae (Z35 & Z29) or from E.coll Z149. However 2 cured cells (2%) were obtained from E.coli Z53 that lost the resistance to ampicillin, tetracycline and chloramphenicol. From treatment with ethidium bromide, four cured cells (4%) were derived from P.aeruginosa Nl that lost the resistance to streptomycin. From P.aeruginosa N3, sixty one cured cells (61 %) were obtained that lost the resistance to different antibiotics like streptomycin, tetracycline, chloramphenicol, gentamycin. Ten cured cells (10%) were also derived from K.pneumoniae Z35 that lost resistance to gentamycin and streptomycin. No cured cells were obtained from treatment of E.coli (Z53 & Z149) and K.pneumoniae Z29 with ethidium bromide. From treatment with acridine orange, three cured cells (3%) were derived from K.pneumoniae Z35 lost the resistance to tetracycline and streptomycin. No cured cells were derived from treatment of other strains with acridine orange. The plasmid curing experiments revealed that: 1 - The effectivness of curing agent does not depend on the bacterial strain only but also on the nature of the plasmid. 2-Large number of antibiotic resistance markers in bacterial strains which had been tested in this study are located on plasmids which can be cured by different curing agents . Results of conjugation experiments between multiresistant bacterial strains (wild type or cured) & E.coli HB101 showed that ampicllin, tetracycline and chloramphenicol resistance markers in E..coli are located on one or more selftransmissible or mobilizable plasmids which are curable by SDS. While streptomycin resistance marker is probably located on another selftransmissible or mobilizable plasmid which can not be eliminated by SDS. Conjugation experiment between cured cells derived from K.pneumoniae and E.coli HB101 revealed that tetracycline and chloramphenicol resistance markers are located on two conjugative plasmids (either selftransmissible or mobilizable) .While streptomycin resistance marker appeared to be carried on nonconjugative plasmid which is curable by ethedium bromide & acridine orange. No conjugation was detected between P.aeruginosa and E.coil.