One hundered patients with verrucous, ulcerative and noduler lesions were included in this study, who attendant Al-Kadhumyia teaching hospital and Baghdad teaching hospital / Dermatology department, (from 4th of May 2008 to the 1st of Febreuary 2009). Thirty six (36) were females and sixty four (64) were males, with the mean age of 48.66 years (ranging between 10-80 years old). And with median of age 47 years. Fifty two patients with ulcerative lesions, fourty six with verrucous and only two patients with noduler ones. The diagnosis was established by clinical examination done by consultant Dermatulogist. Control included 10 appearentlly healthy individuals were randomaly selected as (8 women and 2 men ) with a mean age of 30.3 years (ranging between 20-40 years old ). And for investigation of Blastomyces dermatitidis we used three diagnostic methods:- 1- Cultivation and identification of B.dermatitidis by using sabouraud dextrose agar (SDA) with incubation at 30C for 15 days. Growth was examined grossly and directly under light microscope (X40) by using lactophenol cotton blue. 2- Histopathologically, 5mm-skin biopsies were obtained from each patient from the site of infection preserved immediately in 10% formalin and subsequently embedded in paraffin blocks sectioning. Tissue sections were stained with periodic acid Schiff (PAS) and then exam the budding form (yeast) under light microscope. 3- Immunohistochemistry method was applied for the detection of TGF-β and TNF-α in paraffin embedded tissue sections, from each patient a skin biopsy was obtained from lesion of skin, four micron sections were prepared for subsequent immunohistochemical staining for detection over expression of TGF-β and suppression of TNF–α.The results obtained were:- - We found that eight (8%) percent of those patients were developed blastomycosis according to tissue culture and histopathological exam. 2- The percentage of infection according to over expression of TGF-β and suppression of TNF–α by Immunohistochemistry method was 8%. -