A total of 80 cervical tissue samples were included in this study. 70 archival tissue biopsy samples comprised a risk group for HPV infection and/or cervical neoplasia (20 of condylomatous changes, 9 cases CIN 1, 14 of CIN II/III, and 27 of squamous cell carcinoma).The remaining10 normal cervical post-mortem tissue biopsies were obtained from the Institute of Forensic Medicine and considered as control group. In situ hybridization was performed for the detection of HPV on cervical tissues. Immunohistochemistry was performed to determine the high-risk HPV that actively expressed E6 protein. p53 and p16 were also detected by immunohistochemistry to establish whether expression of these proteins might be useful as a marker for the disease progression and their correlation with HPV status. There is no significant association (p > 0.05) was found between the HPV infection or the expression of E6 protein and the extent of histological abnormalities, but a significant association (p < 0.05) was found between ISH signal pattern & the histological type. 1.A significant correlation (P < 0.01) was found between the expression of p53 & the extent of histological abnormalities. Similarly, A significant correlation was found between p16 expression & the progression of the disease (p < 0.01).
p53 IHC was detected in HPV 16/18 E6 positive & negative cases without significant differences between both groups. But there is a significant difference (P < 0.01) was found in the mean of p16 IHC between HPV-positive & negative groups in CIN II/III & ISCC.