Construction of new Ion Selective electrodes for Determination Chloramphenicol sodium succinate and Iron(III) and their applications in pharmaceutical samples

Two kinds of electrodes were prepared in this study based on PVC matrix. Four ion selective electrodes for Chloramphenicol sodium succinate which based on chloramphenicol palmitate (CPP) and sodium
tetraphenylborate (TPB) as additive, Another Four ion selective electrodes for Iron(III) based on Chloramphenicol sodium succinate- Iron(III) [CPSS-Fe(III)] ion-pair complex as the electro-active material,.
Many plasticizers used: Di-butyl phthalate (DBPH) Di-butyl phosphate (DBP) Di-octyl phthalate (DOP)
Tri-butyl phosphate (TBP) The electrodes parameters were include, linear concentration range, Nernestian slope, limit of detection, response time, life time, working pH rang and selectivity were evaluated. Also the statistical treatments were applied for the results that include: relative standard deviation (RSD), relative error (RE), error and confidence limit for concentration. The results showed: 1- ISES for Chloramphenicol sodium succinate: CPP+TPB+DBPH (membrane A1), CPP+TPB+DBP(membrane A2), CPP+TPB+DOP (membrane A3), CPP+TPB+TBP (membrane A4), gives the slopes (53.98, 51.45, 49.66 and 48.98 mV/decade), linear range from (1x10-4-1x10-1, 5x10-4-1x10-1, 1x10-4-1x10-1, 5x10-4-1x10-1), with detection limit (5x10-5M, 2x10-5 M, 3x10-5 M and 1x10-5 M), response time of 10-3M (15, 18, 20 and 35 second) and the lifetime were about (50, 15, 23 and 21 day ). The working pH ranges were ranged from (2–7.5). the electrode A1 (CPP+TPB+DBPH) has been used to determine Chloramphenicol sodium succinate in the pharmaceutical samples of (Chloramphenicol sodium succinate injection). 2- ISES for Iron(III): CPSS-Fe(III)+DBP (membrane B1), CPSS-Fe(III)+DBPH (membrane B2), CPSS-Fe(III)+DOP (membrane B3) and CPSS-Fe(III)+TBP (membrane B4), gives the slopes (19.79, 26.60, 16.01 and 13.82), linear range from (1x10-5- 1x10-2 M, 1x10-5-1x10-2 M, 1x10-6-1x10-2 M and 1x10-5-1x10-2 M), with detection limit (9×10-6 M, 7×10-5 M, 2×10-6 M and 9×10-5 M), response time of 10-3 M (10, 35, 15, 35 and 30 second), lifetime were about (37, 41, 23 and 16 days). The working pH ranges were ranged from (2 - 6) by using electrode (CPSS-Fe(III)+DBP), and this electrode has been used to determine Iron(III) in the pharmaceutical samples of (Feroglobin Capsules). The selectivity coefficients (Kpot A,B) of ISES have been studied for the following interference ions (Na+, K+, Mn+2, Cu+2, Zn+2, Fe+3, Al+3, Chloramphenicol palmitate, folic acid , Sucrose and Gelatin) by using separate solution method and fixed interfering mixed method. The UV-spectrophotometric method which includes: - The derivative spectra, the first-derivative (1D) spectra for Chloramphenicol sodium succinate solutions (2-64 mg/L) in wavelength equal 258 nm with (r2=0.99925). The analytical methods results showed to be simple, rapid and with a good accuracy by comparing between First Derivative (1D) and direct method of Ion selective electrode by using Ftest. The results shown, that the Chloramphenicol sodium succinate can be determined by using Ion selective electrode method because the value of the (F) experimental less than the value of the (F) theoretical at 95%
confidence limit. Since Fcalculated‹ Ftable, we concluded that there is no significant difference in precision between two methods.