Spermidine, spermine and trace elements levels in serum of breast cancer patients

In this study, serum polyamine (spermidine & spermine) and some essential trace elements (copper, zinc, iron, chromium, manganese, magnesium, cobalt, and selenium) levels were measured in 58 breast cancer (BRCA) patients and compared with 25 healthy controls and to search of possible biochemical marker and causes of
breast cancer, attention was paid to the role played by polyamine and trace element in breast cancer. Blood samples from two groups (control & patients) were prepared and quantitative determination of polyamine and trace element were determined polyamine by using High Performance Liquid Chromatography (HPLC), while
the trace elements, copper, zinc, iron, chromium, manganese, magnesium, and cobalt determine by using Flame Atomic Absorption Spectrophotometer, While selenium was determined by Flameless Atomic Absorption Spectrophotometer. It is found that spermidine a significantly increase (p<0.05) in patients levels was observed when breast cancer patient compare with normal subjects, and significantly increase than the healthy control population to the spermine with (p<0.05). Copper concentration was higher in breast cancer patients, while iron, zinc, magnesium, manganese, selenium, chromium, and cobalt levels have shown a remarkable decrease in those patients when compared to the control group, and the metal to metal ratio measured in this work is very indicative in the studied, while serum Cu/Zn level significantly increase than the healthy controls subjects.