Determination of Carbamazepine in human serum using second derivative absorption spectroscopy.

number: 
283
إنجليزية
department: 
Degree: 
Imprint: 
Chemistry
Author: 
Tariq Muhsin Haider
Supervisor: 
Dr.Yahya Y. Zaki Farid
Dr.shahbaz A.Maki
year: 
1998

Abstract:

Derivative spectrometry technique has been used for the measurement oF carbamazepine in human strum for patients receiving this drug. Second derivative spectrum at 258 nm was chosen for this purpose. Blood sera from forty patients under carbamazepine therapv, Forty five normal peoples used as control, as well as other volunteers were examined in this study. The method has been also used for the determination of the drug concentration For all the above groups. Interferences clue to the presence of other drugs, that are given concurrently, were not observed at the Selected wavelength. This method has also been used for the measurement of some pharmacokinctic parameters. The calculated half-lives, volume of distribution, and the rate of clearance of drug were in good agreement with the reported data using other techniques. In addition, the side effects of the drug on liver and renal functions were studied. No significant evidence For liver and renal dysfunction was observed. The biochemical tests For patients using this drug were within the normal ranges. However, sugar, urea, ceratinine, GPT, and ALP mean values were Found to be higher for the patients than that of the control persons (p < 0.05)The calibration curve used for this method this a wide linear range of 2 to 30 ug/ml of carbarbamazcpinc concentration. The relative standard deviation of the slop was 1.34 % with correlation coefficient of 0.9993 at 95% confidence limit.