This study included extraction of total flavonoids compounds from Matricaria chamomile flowers that grown in Iraq. The extract was brown color crystals composition. Querecetin and rutin were isolated by TLC, HPLC and identified by using UV-Visible spectra and FTIR spectra . HPLC method used to separated and identified another flavonoid compounds such as apigenin, catechin, myrectin, other poly(phenolic) compounds were separated such as coumarin, scopoletin, umbelliferone, tannic acid, gallic acid . Also the local market chamomile flowers extract examined by TLC method using same solvent systems, viewed that local market flowers extract was free from flavonols querectin and rutin. The concentration of metals found in plant material was determined using atomic absorption spectrophotometer .