This work presents the design and construction of an experimental arrangement to detect the fluorescence spectrum and measure decay lifetime of solid state laser media that emits at 1.06 jim wavelength, and the implementation of computer software to determine the spectroscopic characteristics of the laser media under test. Five Nd:glass samples have been used, with doping ratios of 2%,3%, and 6% of Neodymium ion in two types of hosts are Borate and Borophosphate. The experimental work is based on using CW diode laser (810 nm) as the pumping source,anoptical filter at 1.06 um and a photodetecter for signal detection. The absorption spectra for the above samples have been measured by using the Lambda-9 spectrophotometer, from which the peak absorption cross-section, the integrated absorption cross-section for all absorption bands, and the bsoiption line strength and oscillator strength have been calculated.The above results have then been used to calculate the fluorescence line strength, spontaneous emission probability, and radiative lifetime using Judd-Ofelt analysis. The emission quantum efficiency and the non-radiative decay rate have been calculated using the results of measurements and calculations above.