Evaluation the effect of some plant active compounds on bacterial plasmid.+CD

number: 
2337
إنجليزية
Degree: 
Imprint: 
Biotechnology
Author: 
Heba Mohammad Jwad Ahmed
Supervisor: 
Dr. Khulood W. Al-Samarraei
Dr. Hameed M. Jasim
year: 
2009
Abstract:

Aqueous and methanolic extracts of dried aerial parts of Hibiscus sabdariffa and Harpagophytum procumbens were tested for their antimicrobial and curing activity against two microorganisms Escherichia coli HB 101 and seudomonas aeruginosa RB19 . Chemical investigation on these extracts showed that Hibiscus sabdariffa methanolic extracts contain flavonoids and water extract contain glycosides and Saponins while the Harpagophytum procumbens methanolic extracts contain flavonoids and water extract contain glycosides. The in vitro Results showed that water and methanolic extracts of Hibiscus sabdariffa and methanolic extract of Harpagophytum procumbens have antimicrobial effect against E.coli HB 101 and P. aeruginosa RB19, while aqueous extract of Harpagophytum procumbens have an antimicrobial effect against P. aeruginosa RB19 but do not have any effect against E. coli HB 101. The sensitivity of P. aeruginosa RB 19 and E.coli HB 101 against different antibiotics was examined, and it was found that E.coli HB 101 was able to resist ampicillin and tetracycline, while it was sensitive to erythromycin, rimethoprim, chloromphenicol, streptomycin, fusidic acid, vancomycin, imipenem, cephotaxime, cephalothin, cephalexin, gentamicin, amoxycillin, penicillin G, ticarpen mand clavulanic acid. While P. aeruginosa RB19 was resistant to ampicillin, amoxycillin, chloromphenicol, penicillin G, cephalothin, cephotaxime, tetracycline, and clavulanic acid, while it was sensitive to erythromycin, trimethoprim, gentamicin, streptomycin, Fusidic acid, ticarpen, cephalexin, vancomycin, and imipenem. Plasmid profile of P. aeruginosa RB19 and E. coli HB 101 was studied by extraction the total DNA according to alkaline lysis method, then electrophoresis on agarose gel. Results showed that E.coli HB 101 strain was harburing a single small plasmid, while in P.aeruginosa RB 19 isolate, there are two plasmids( large and small). Curing of plasmid DNA was achieved using Ethidium bromide to know the role of this plasmid in the virulence and its ability to antibiotic resistance. Results showed that there were a number of P. aeruginosa RB 19 colonies had lost their resistance to tetracycline, ampicillin and cephalothin. While those of E. coli HB 101 lost their resistance to ampicillin and tetracycline. Curing of plasmid DNA was achieved by treating P. aeruginosa RB 19 and E.coli HB 101 with Hibiscus sabdariffa and Harpagophytum procumbens extracts showed that many of these colonies were lost their resistance to antibiotics. These results indicated that extracts of those plants were have a curing effect on the bacterial plasmids. Hibiscus sabdariffa methanolic extract was the best extract that caused E.coli HB 101 to loss resistance to ampicillin and tetracycline and Harpagophytum procumbens methanolic extract caused P. aeruginosa RB 19 to loss resistance to ampicillin, tetracycline and cephalothin. .