Inhibition of adherence of proteus mirabilis to uroepithelial cells by sub minlmum inhibitory concentrations of antibiotics.

number: 
724
إنجليزية
Degree: 
Imprint: 
Biotechnology
Author: 
Melad Mumtaz Abdul Al-Ahad Al-Samak
Supervisor: 
Dr.Subhi J. Hamza
Dr. Mohamad A.K. Ibrahim
year: 
2003
Abstract:

Specimens from both sexes and different ages were collected from Medical University Hospital of Saddam College of medicine, Al-Yarmoq Hospital and AL-Karama Hospital. Out of (600) urine samples which were collected from patients expected to have urinary tract infection, (478) samples showed positive result in culture. From the positive result (56) sample were identified as Proteus (11.7%) .Isolation percentage of Proteus in samples taken from males was more than in females; as 71.4% and 28.6% respectively. P.mirabilis was the most common pathogen of UTI ,with isolation percentage 80.35% as compared to that of P.vulgaris which was 19.6%. Susceptibility tests toward twenty antibiotics were carried out. Results shows that ceftazidin ,amikicin and ciprofloxacin were the most effective antibiotics and their resistance percentage were 8.8%,11% and 11% respectively. While flucloxacillin ,erythromicin and rifampicin were not effective and their resistance percentage was 100%. Eight isolates were selected depending on the result of antibiotic sensitivity tests as showed multi-antibiotic resistance and tested to specify their minimum inhibitory concentration for amoxicillin, cephalexin ,amikicin ,gentamicin ,naldixic acid, ciprofloxacin, erythromycin and rifapecin. Ciprofloxacin and amikicin were found to have the lowest MIC as compared with other antibiotics. Eight isolates were selected and tested for adherence ability to uroepithelium from normal female. All isolates were found to adhere to uroepithelium .However; isolate number 7 had the highest ability of adherence with the mean number of adhering bacteria equivalent to (33.3) bacteria per uroepithelium as compared with other isolates. Sub MICs effect (1/4, 1/2, 3/4 MIC) of amoxicillin, cephalexin ,amikicin ,gentamicin ,naldixic acid ciprofloxacin, erythromycin and rifapecin on the adherence ability of eight isolates were determined. The obtained results indicated that amikicin, gentamycin and ciprofloxacin were the most effective antibiotics as compared with others while cephlexin has no effect on the adherence ability of P.mirabilis. Isolation and purification of pilin from isolate number 7 treated and with out treated with (1/4, 1/2, 3/4 MIC) of amikacin and ciprofloxacin was done. SDS polyacrylamid gel electrophresis profile of pilin indicate that amikacin and ciprofloxacin at sub MICs was found to have a significant effect on the reduction of pilin production.